Description
Acetoacetate (AAT), a β-ketoacid, is one of the three ketone bodies and is formed via condensation of two molecules of acetyl-CoA in liver mitochondria. AAT can be enzymatically reduced to 3-β-hydroxybutyrate (β-HB), or decarboxylated producing acetone ((CH₃)₂CO). Ketone bodies [ β-HB: 78%; AAT: 20% & (CH₃)₂CO: 2% ] are mainly used as an alternative energy source when glucose cannot be delivered to the system. Excessive concentration of ketone bodies (ketoacidosis) is observed in patients with Type I diabetes, severe starvation or alcoholism. Simple, direct and automation-ready procedures for measuring acetoacetate (AAT) are very desirable. MesGen Biotech' Acetoacetate assay is based on a reaction with Sodium Nitroprusside forming a product with absorbance at 550 nm.

Applications
Direct assay of acetoacetate in serum, plasma, urine and other biological samples.

Key Features
Sensitive and accurate. Linear detection range of 0.005 to 1 mM for acetoacetate in 96-well plate assay.
Note: Considering the influence of acetone interfere, if the AAT concentration in sample is >1 mM, please dilute sample in H₂O and repeat this assay.
Convenient. The procedure involves adding a single working reagent and reading the optical density at room temperature in kinetic mode.
High-throughput. Can be automated as a high-throughput 96-well plate assay for thousands of samples per day.

Storage conditions
The kit is shipped at dry ice. Store all reagents at -20°C.

Shelf life
6 months after receipt, the Working Reagent of reconstitution should be stored at -80°C and used within 1 month.

产品仅供科学研究 禁止用于临床诊断、治疗
For Research Use Only. Not For Use In Diagnostic Procedures