Description
L-Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. MesGen Biotechnology L-lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which reduces a probe into a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the galactose concentration in the sample.

Application
Direct Assays: L-lactate in serum, plasma, urine, cell media samples and other biological samples.

Key features
Sensitive and accurate. Detection limit of 2 μM and linearity up to 500 μM L-lactate in 96-well plate assay.
Convenient. The procedure involves adding a single working reagent, and reading the colorimetric or fluorescence after 5-10 min. Room temperature assay.
High-throughput. Can be automated as a high-throughput 96-well plate assay for thousands of samples per day.

Storage conditions
The kit is shipped on ice. Store all reagents at -20°C.

Shelf life
6 months after receipt.

产品仅供科学研究 禁止用于临床诊断、治疗
For Research Use Only. Not For Use In Diagnostic Procedures.