Description
Choline and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety. MesGen Biotechnology' method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to betaine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570 nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.

Key Features
Use 20 μL samples. Linear detection range: colorimetric assay 0.6 to 150 μM, fluorimetric assay 0.1 to 15 μM choline.

Applications
Assays: choline in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture.
Drug Discovery/Pharmacology: effects of drugs on choline metabolism.

Storage conditions
The kit is shipped on dry ice. Store all components at -20°C.

Shelf life
6 months after receipt.

产品仅供科学研究 禁止用于临床诊断、治疗
For Research Use Only. Not For Use In Diagnostic Procedures.