Description
Glycogen is a branched polysaccharide of glucose units linked by α-1,4 glycosidic bonds and α-1,6 glycosidic bonds. It is stored primarily in the liver and muscle, and forms an energy reserve that can be quickly mobilized to meet a sudden need for glucose. The most common glycogen metabolism disorder is found in diabetes, in which, due to abnormal amounts of insulin, liver glycogen can be abnormally accumulated or depleted. Genetic glycogen storage diseases have been associated with various inborn errors of metabolism caused by deficiencies of enzymes necessary for glycogen synthesis or breakdown. Simple, direct and automation-ready procedures for measuring glycogen concentrations find wide applications in research and drug discovery. MesGen Biotechnology ' glycogen assay uses a single Working Reagent that combines the enzymatic break down of glycogen and the detection of glucose. The color intensity of the reaction product at 570nm or fluorescence intensity at lex/em = 530/585 nm is directly proportional to the glycogen concentration in the sample. This simple convenient assay is carried out at room temperature and takes only 30 min.

Key Features
Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 4 to 2000 µg/mL glycogen for colorimetric assays and 0.4 to 200 µg/mL for fluorimetric assays.
Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5-10 min at room temperature.

Storage conditions
The kit is shipped on dry ice. Store all components at -20°C.

Shelf life
6 months after receipt.

产品仅供科学研究 禁止用于临床诊断、治疗
For Research Use Only. Not For Use In Diagnostic Procedures.