Description
Mes-Red Hydrogen Peroxide/Peroxidase Assay Kit could use the Mes-Red reagent to detect hydrogen peroxide (H₂O₂) or peroxidase activity. The Mes-Red reagent, in combination with horseradish peroxidase (HRP), has been used to detect H₂O₂ released from biological samples, including cells, or generated in enzyme-coupled reactions. Furthermore, Mes-Red reagent can be used as an ultrasensitive assay for peroxidase activity when H₂O₂ is in excess. In the presence of peroxidase, the Mes-Red reagent reacts with H₂O₂ in a 1:1 stoichiometry to produce the red-fluorescent oxidation product, resorufin. Resorufin has excitation and emission maxima of approximately 570 nm and 585 nm, respectively, and because the extinction coefficient is high, you can perform the assay fluorometrically or spectrophotometrically. The optical density (570 nm) or fluorescence intensity (lex/em=530/585 nm) is a direct measure of the enzyme activity. This reaction has been used to detect as little as 10 picomoles of H₂O₂ in a 100 μL volume (50 nM) or 1 × 10^–5 U/mL of HRP.

Storage condition
-20°C & Protected from light. Shelf life: 6 months after receipt.
 
产品仅供科学研究 禁止用于临床诊断、治疗
For Research Use Only. Not For Use In Diagnostic Procedures.