Description
Cholesterol is a sterol and lipid present in the cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones, and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; whereas, low levels (hypocholesterolemia) may be linked to depression, cancer and cerebral hemorrhage. Simple, direct and automation-ready procedures for measuring cholesterol are very desirable. MesGen Biotechnology' Cholesterol Assay uses a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at lem/ex = 585/530nm is directly proportional to total cholesterol concentration in the sample.

Applications
Direct Assays: cholesterol in serum, plasma, and other biological samples.
Pharmacology: effects of drugs on cholesterol metabolism.

Key Features
Sensitive and accurate. Linear detection range in 96-well plate: 0.4 to 100 μg/mL cholesterol for colorimetric assays and 0.1 to 10 μg/mL for fluorimetric assays.
Convenient. Room temperature assay. No 37°C heater is needed.
High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Storage conditions
The kit is shipped on dry ice. Store reagents at -20°C.

Shelf life
6 months after receipt.

产品仅供科学研究 禁止用于临床诊断、治疗
For Research Use Only. Not For Use In Diagnostic Procedures.