硫黄素T染色液 (2.5mM) Thioflavin T Stain Solution (2.5mM)

产品中心

产品货号

产品名称

CAS号

包装

市场价（人民币）

库存状态

MST8538

硫黄素T染色液 (2.5mM) Thioflavin T Stain Solution (2.5mM)

100ml

210.00

In stock

经销商：您可以直接联系本地区的授权代理商

Email：sales@mesgenbio.com

产品概述
Description Thioflavin T (ThT) is a benzothiazole salt obtained by the methylation ofdehydrothiotoluidine with methanol in the presence of hydrochloric acid. ThT is used as a dye to visualize and quantify the presence or fibrilization of misfolded protein aggregates, or amyloid, both in vitro and in vivo (e.g.,plaques composed of amyloid beta found in the brains of Alzheimer's disease patients; plaques of PrP fibrils found in brains of CGD patients). The Thioflavin T (ThT) Assay measures changes of influorescence intensity of ThT upon binding to amyloid fibrils. The enhanced fluorescence can be observed by fluorescence microscopy or by fluorescent spectroscopy. The spectroscopic assay is commonly used to monitor fibrilization over time, but the assay is not strictly quantitative and differences in binding have been observed for samples after lyophilization. Protocol 1. Dilute the stock solution into the phosphate buffer (final 5-50uM) on the day of analysis to generate the working solution. 2. Measure the fluorescence intensity of 1mL working solution by excitation at 440 nm (slitwidth 5 nm) and emission 482 nm (slitwidth 10 nm), averaging over 60 s. 3. Add an aliquot of untreated protein solution (5–10μL) to the cuvette, stir for 1 min, and measure the intensity over 60 s. This serves as the control sample. 4. Repeat steps 3–4 with 5–10µL of the aggregated protein solution. A measured intensity above the control sample is indicative of amyloid fibrils. Note: this ThT assay protocol is for testing in a cuvette,and is valid if you use multi-scan spectrometer that requires multi-well plates. 产品简介硫黄素 T (ThT) 是一种苯并噻唑染料，在与淀粉样蛋白原纤维结合后表现出增强的荧光，通常用于离体和体外诊断淀粉样蛋白原纤维。在水溶液中，发现硫黄素 T 以胶束形式存在，其浓度通常用于通过荧光测定法监测原纤维 (~10 -20 μM)。在硫黄素 T 的不同浓度下测量比电导率变化，临界胶束浓度计算为 4.0±0.5 μM。硫黄素 T 的荧光激发和发射的变化也取决于胶束的形成。使用原子力显微镜直接观察直径为 3 nm 的硫黄素 T 胶束，并沿着纤维长度观察具有代表性的原纤维的结合硫黄素 T 胶束。硫黄素 T 的浓度增加到临界胶束浓度以上表明沿着淀粉样原纤维长度结合的胶束数量增加。硫黄素 T 胶束在低 pH 值下被破坏，如通过原子力显微镜观察到的，硫黄素 T 与淀粉样蛋白原纤维结合后的荧光增强在 pH 值降至 3 以下时也减少了几倍。硫黄素 T 的胶束结合淀粉样蛋白原纤维导致增强荧光发射。保存条件 2-8°C，避光保存，有效期6个月。产品仅供科学研究禁止用于临床诊断、治疗 For Research Use Only. Not For Use In Diagnostic Procedures

产品概述

Description

Thioflavin T (ThT) is a benzothiazole salt obtained by the methylation ofdehydrothiotoluidine with methanol in the presence of hydrochloric acid. ThT is used as a dye to visualize and quantify the presence or fibrilization of misfolded protein aggregates, or amyloid, both in vitro and in vivo (e.g.,plaques composed of amyloid beta found in the brains of Alzheimer's disease patients; plaques of PrP fibrils found in brains of CGD patients). The Thioflavin T (ThT) Assay measures changes of influorescence intensity of ThT upon binding to amyloid fibrils. The enhanced fluorescence can be observed by fluorescence microscopy or by fluorescent spectroscopy. The spectroscopic assay is commonly used to monitor fibrilization over time, but the assay is not strictly quantitative and differences in binding have been observed for samples after lyophilization.

Protocol

1. Dilute the stock solution into the phosphate buffer (final 5-50uM) on the day of analysis to generate the working solution.

2. Measure the fluorescence intensity of 1mL working solution by excitation at 440 nm (slitwidth 5 nm) and emission 482 nm (slitwidth 10 nm), averaging over 60 s.

3. Add an aliquot of untreated protein solution (5–10μL) to the cuvette, stir for 1 min, and measure the intensity over 60 s. This serves as the control sample.

4. Repeat steps 3–4 with 5–10µL of the aggregated protein solution. A measured intensity above the control sample is indicative of amyloid fibrils.

Note: this ThT assay protocol is for testing in a cuvette,and is valid if you use multi-scan spectrometer that requires multi-well plates.

产品简介

硫黄素 T (ThT) 是一种苯并噻唑染料，在与淀粉样蛋白原纤维结合后表现出增强的荧光，通常用于离体和体外诊断淀粉样蛋白原纤维。在水溶液中，发现硫黄素 T 以胶束形式存在，其浓度通常用于通过荧光测定法监测原纤维 (~10 -20 μM)。在硫黄素 T 的不同浓度下测量比电导率变化，临界胶束浓度计算为 4.0±0.5 μM。硫黄素 T 的荧光激发和发射的变化也取决于胶束的形成。使用原子力显微镜直接观察直径为 3 nm 的硫黄素 T 胶束，并沿着纤维长度观察具有代表性的原纤维的结合硫黄素 T 胶束。硫黄素 T 的浓度增加到临界胶束浓度以上表明沿着淀粉样原纤维长度结合的胶束数量增加。硫黄素 T 胶束在低 pH 值下被破坏，如通过原子力显微镜观察到的，硫黄素 T 与淀粉样蛋白原纤维结合后的荧光增强在 pH 值降至 3 以下时也减少了几倍。硫黄素 T 的胶束结合淀粉样蛋白原纤维导致增强荧光发射。

保存条件

2-8°C，避光保存，有效期6个月。

产品仅供科学研究禁止用于临床诊断、治疗

For Research Use Only. Not For Use In Diagnostic Procedures

产品手册	MSDS	COA	参考

产品中心

欢迎给我们留言