Description
Transforming growth factor beta 1 (TGFβ1) is the prototype of a growing superfamily of peptide growth factors and plays a prominent role in a variety of cellular processes, including cell-cycle progression, cell differentiation, reproductive function, development, motility, adhesion, neuronal growth, bone morphogenesis, wound healing, and immune surveillance. TGF-β1, TGF-β2 and TGF-β3 signal via the same heteromeric receptor complex, consisting of a ligand binding TGF-β receptor type II (TβR-II), and a TGF-β receptor type I (TβR-I). Signal transduction from the receptor to the nucleus is mediated via SMADs. TGF-β expression is found in cartilage, bone, teeth, muscle, heart, blood vessels, haematopoitic cells, lung, kidney, gut, liver, eye, ear, skin, and the nervous system.

Product origin
Recombinant Mouse/Rat Transforming Growth Factor beta 1 is produced by our Mammalian expression system and the target gene encoding Ala279-Ser390 is expressed. 

Dissolution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 4mM HCl. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Store condition
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Formulation
Lyophilized from a 0.2 μm filtered solution of 4mM HCl.

Quality control
Purity : Greater than 95% as determined by reducing SDS-PAGE.   
Endotoxin : Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity : Measured by its ability to inhibit IL-4-dependent proliferation of TF-1 human erythroleukemic cells.

For Research Use Only. Not For Use In Diagnostic Procedures