Background 
GAPDH is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. This GAPDH antibody can be used as a loading control antibody. There is no cross-reaction with GAPDH from yeast. Preliminary data indicates that the antibody recognizes the monomer (36 kDa) and also the dimer forms of GAPDH, but not the tetrameric form of the protein.

Overview
Description : Mouse monoclonal antibody to GAPDH [3G7] 
Reactivity : Mouse, Rat, Human
Tested applications : ELISA, ICC, WB
Formulation : Liquid

Properties
Immunogen : This mouse monoclonal antibody is produced by immunizing mouse with Full length native protein from human erythrocytes.
Clonality : 3G7
Isotype : Mouse IgG1

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows:
WB (1:2000-1:10000)
IHC (1:50-1:200)
IF/ICC (1:50-1:200)
 
Storage instruction
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C

For Research Use Only. Not For Use In Diagnostic Procedures.