Product Description
M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten's Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently. M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator. Biological Performance: This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.

Storage/Stability
Store the liquid medium at 2–8 °C in the dark. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date. Deterioration of the liquid medium may be recognized by any of the following:
1. pH change
2. cloudy appearance
3. color change.
Note: Slight precipitate or particulate matter may be seen throughout the solution, which does not affect the performance of the product.

For Research Use Only. Not For Use In Diagnostic Procedures.